Stability of cisplatin, doxorubicin, and mitomycin combined with loversol for chemoembolization

OBJECTIVE: To evaluate the chemical stability of a mixture of cisplatin 10 mg/mL, doxorubicin 5 mg/mL, and mitomycin 1 mg/mL in sodium chloride 0.9% (NS), and sodium chloride 0.9% and ioversol 68% (NSI). METHODS: The agents were reconstituted with NS and NSI in two groups. One syringe was made and stored protected from light at 4, 25, and 37 degrees C. Triplicate HPLC determinations were performed on each syringe at each time period. Drug concentration at T0, 4, 12, 24, 72, and 120 hours were recorded. RESULTS: Cisplatin in NS remained stable for five days at all three temperatures and lost 12.7% at 37 degrees C in 120 hours in NSI. Doxorubicin in NS remained stable throughout the five days at 4 and 25 degrees C, but lost 13.1% at 37 degrees C in 24 hours. Doxorubicin in NSI remained stable for five days at 4 degrees C and lost 16.3% at 25 degrees C by day 5, and 18.8% at 37 degrees C in 24 hours. Mitomycin in NS lost 15.8% at 4 degrees C within 24 hours, and 24.7% at 25 degrees C and 36% at 37 degrees C within four hours. Mitomycin in NSI was stable for 72 hours at 4 degrees C, 24 hours at 25 degrees C, and less than four hours at 37 degrees C. CONCLUSIONS: These agents in NS are stable for 12 hours at 4 degrees C. In NSI, they are stable for 72 hours at 4 degrees C.